ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) : ASCP-MLT Exam

  • Exam Code: ASCP-MLT
  • Exam Name: MEDICAL LABORATORY TECHNICIAN - MLT(ASCP)
  • Updated: Aug 10, 2026
  • Q & A: 562 Questions and Answers

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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Topic 1: Microbiology15–20%- Bacteriology and mycology procedures
- Virology and parasitology testing
Topic 2: Blood Banking15–20%- Blood group systems and immunology
- Blood products and components
- Transfusion practice and testing
Topic 3: Immunology5–10%- Serologic procedures and immune diseases
- Principles of immunology
Topic 4: Hematology20–25%- Physiology and disease states
- Laboratory testing and hemostasis
Topic 5: Laboratory Operations5–10%- Instrumentation and laboratory mathematics
- Quality assessment and safety
Topic 6: Chemistry20–25%- Carbohydrates, lipids, and enzymes
- Electrolytes, acid-base, and toxicology
Topic 7: Urinalysis and Other Body Fluids5–10%- Microscopic analysis and disease correlation
- Physical and chemical testing

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Francisella is slow growing on primary isolation culture media and produces poorly-staining coccobacilli on Gram stain. Because Francisella tularensis is included on the list of bioterrorism agents, suspicious isolates should be referred immediately to a public health laboratory in lieu of attempting an in-laboratory identification.
One of the BIGGEST problems with isolating Francisella is that these organisms:

A) grow so slowly
B) produce greenish colonies like E. coli
C) overgrow other organisms
D) produce wide zones of hemolysis


2. Serum separator tubes should not be used for:
Question options:

A) therapeutic drug tests
B) blood bank tests
C) serology tests
D) all of the above


3. Transmission-based precautions isolation categories include all of the following except:

A) airborne.
B) blood and body fluids.
C) droplet.
D) contact.


4. The blood glucose value is critical; negative ketones is consistent with type 2 diabetes. An elderly type 2 diabetic patient is at risk for hyperosmolar non-ketotic coma and osmolality should be measured.
A measurement of urine glucose would not be useful at this point.
A 70-year-old type 2 diabetic patient is comatose and in the emergency department. Stat blood glucose and serum ketones are ordered.
Blood Glucose: 650 mg/dL
Serum Ketones: Negative
What is the best conclusion?

A) The glucose result is critical and the patient should be evaluated for hyperosmolality
B) Glucose result is acceptable for type 2 diabetic patient
C) Urine glucose should be measured
D) Because of the glucose and ketone result, the patient now has type 1 diabetes


5. Spirochetes such as Borrelia and Treponemes are best visualized using darkfield microscopy. Borrelia, which causes Relapsing fever, can be visualized under darkfield microscopy of wet preps of peripheral blood.
Treponema can be visualized by darkfield microscopy of early primary lesions, and aspirates of affected lymph nodes.
Which of the following organisms is best visualized by use of a darkfield microscope:

A) Borrelia
B) Streptococcu
C) Campybacter
D) Entamoeba


Solutions:

Question # 1
Answer: A
Question # 2
Answer: D
Question # 3
Answer: B
Question # 4
Answer: A
Question # 5
Answer: A

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